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Photosynthesis and carbon metabolism in plantain (Musa AAB) plantlets growing in temporary immersion bioreactors and during ex vitro acclimatization

Authors :
Sophie Roels
Maritza Escalona
Danilo Pina
Pierre Debergh
Iris Capote
Justo González-Olmedo
J. Sandoval
Roberto Rodríguez
Carlos Aragón
Inaudis Cejas
María Jesús Cañal
Source :
In Vitro Cellular & Developmental Biology - Plant. 41:550-554
Publication Year :
2005
Publisher :
Springer Science and Business Media LLC, 2005.

Abstract

The photosynthetic capacity changes and the main enzymatic systems related to carbon metabolism were investigated during the in vitro culture of plantain shoots (Musa AAB cv. CEMSA 3/4) in temporary immersion bioreactors (TIB) and their subsequent acclimatization. The maximal rate of photosynthesis (Pn), transpiration, and the activity of the carbon metabolism enzymes phosphoenolpyruvate carboxylase (PEPC), acid invertase (AI), pyruvate kinase (PK) and sucrose phosphate synthase (SPS) were measured every 7 d during the 21 d of elongation in TIB, and the following 42 d of acclimatization. Sucrose content in the liquid medium and in the leaves was also determined. The most significant changes in plant growth were observed during acclimatization. During the in vitro stage photosynthesis was limited (4–6 μmol CO2 m−2 s−1); the photosynthetic rate however increases rapidly and significantly as soon as in vitro culture is over during acclimatization. PEPC activity increased during the whole evaluation ...

Details

ISSN :
14752689 and 10545476
Volume :
41
Database :
OpenAIRE
Journal :
In Vitro Cellular & Developmental Biology - Plant
Accession number :
edsair.doi...........0ea87c7e24674d3faf12abdba80760ce
Full Text :
https://doi.org/10.1079/ivp2005640