Novel 3D Printed Microfluidic Paper-Based Analytical Device With Integrated Screen-Printed Electrodes for Automated Viscosity Measurements

Various miniaturized viscometers have been developed utilizing several fabrication methods. Among them, microfluidic paper-based analytical devices ( $\mu $ PADs) are becoming popular due to their fabrication ease, cost-effectiveness, and the fact that the flow can be carried out using the embedded capillaries themselves. Mostly, $\mu $ PADs are reported to be fabricated by a solid-ink printer, which has significantly high capital and operational cost. To overcome such drawbacks, a novel rapid prototyping method has been proposed, wherein the formation of the hydrophobic regions was created by polycaprolactone (PCL) filament using a 3-D printer. To leverage this, $\mu $ PAD as a viscometer, velocity, or time between two points with known distances was required, which was carried out by an amperometric approach, established by fabricating the integrated screen-printed electrodes intersecting the microchannel of the $\mu $ PAD. The time measurement was fully automated by a microcontroller, and the relative viscosity was calculated by comparing the time taken by the reference fluid with that of a test fluid to cover a known length. Such integrated, automated, and low-cost paper-based microviscometer was leveraged to measure and analyze the viscosities of various milk variants, which has an accuracy of >92%.