Effects of culture conditions on in vitro bulblet induction in the medicinal plant Amana edulis (Miq.) Honda

Amana edulis (Miq.) Honda is an important medicinal plant with a variety of anti-cancer properties, and it is of great importance to improve its reproductive rate through micropropagation technology to meet increasing demand. In the present study, in order to establish a rapid in vitro bulblet propagation protocol for A. edulis, an L16 (45) orthogonal design was used to investigate the effects of sucrose, 6-benzyladenine (6-BA), α-naphthaleneacetic acid (NAA), and macro-elements on bulblet induction. The results show that the sucrose concentration was the crucial factor for A. edulis bulblet initiation, followed by 6-BA and macro-elements, while NAA had the weakest effect. The optimal medium for in vitro bulblet induction was Murashige and Skoog medium supplemented with 0.1 mg·L−1 6-BA, 0.01 mg·L−1 NAA, and 100 g·L−1 or 80 g·L−1 sucrose (pH 5.8), in which A. edulis shoot clusters (without roots) were cultured at 5(±2)°C for 35 d or 60 d and then incubated at 23(±2)°C for 90 d. The entire cultivati...