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Enzymatic profiling of cellulosomal enzymes from the human gut bacterium,Ruminococcus champanellensis, reveals a fine-tuned system for cohesin-dockerin recognition
- Source :
- Environmental Microbiology. 18:542-556
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Ruminococcus champanellensis is considered a keystone species in the human gut that degrades microcrystalline cellulose efficiently and contains the genetic elements necessary for cellulosome production. The basic elements of its cellulosome architecture, mainly cohesin and dockerin modules from scaffoldins and enzyme-borne dockerins, have been characterized recently. In this study, we cloned, expressed and characterized all of the glycoside hydrolases that contain a dockerin module. Among the 25 enzymes, 10 cellulases, 4 xylanases, 3 mannanases, 2 xyloglucanases, 2 arabinofuranosidases, 2 arabinanases and one β-glucanase were assessed for their comparative enzymatic activity on their respective substrates. The dockerin specificities of the enzymes were examined by ELISA, and 80 positives out of 525 possible interactions were detected. Our analysis reveals a fine-tuned system for cohesin-dockerin specificity and the importance of diversity among the cohesin-dockerin sequences. Our results imply that cohesin-dockerin pairs are not necessarily assembled at random among the same specificity types, as generally believed for other cellulosome-producing bacteria, but reveal a more organized cellulosome architecture. Moreover, our results highlight the importance of the cellulosome paradigm for cellulose and hemicellulose degradation by R. champanellensis in the human gut.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
biology
Cohesin
Dockerin
Cellulase
biology.organism_classification
Microbiology
Cellulosome
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Enzyme
chemistry
Biochemistry
biology.protein
Glycoside hydrolase
Cellulose
Ecology, Evolution, Behavior and Systematics
Bacteria
Subjects
Details
- ISSN :
- 14622912
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi...........0e5fb1ad6044344ed1ee03f9fad2f75a