Bioinformatics and Expression Analysis of GmWRKY32 Gene in Soybean

WRKY transcription factors are very important transcriptional regulators in the process of plant response to plant growth and abiotic stresses. In this study, the open reading frame (ORF) of soybean GmWRKY32 gene was cloned from the WRKY sequence of soybean genome, which was a polypeptide encoding 300 amino acids. Bioinformatics analysis revealed that the WRKY32 protein contained a highly conserved WRKY domain and belonged to Group III of the WRKY gene family. The homology with Glycine soja GsWRKY70, Cajanus cajan CcWRKY70 and Vigna angularis VaWRKY70 proteins was 85%, 79% and 72%, respectively. In addition, GmWRKY32 , is an insoluble protein, no signal peptide and transmembrane domain. Further analysis of the gene expression pattern under different stress and hormone induction showed that after 12 h of treatment with PEG, high salt stress and JA, the expression of GmWRKY32 was increased by 4.5, 4.2 and 3.2 times than that of control, respectively. After 24 h of ABA induction, the expression level of GmWRKY32 was increased 6-fold compared with that of the control. Conjecture that it is closely related to the resistance of soybean to abiotic stress which would lay a foundation for the family structure and functional research of soybean WRKY transcription factors, and will provide scientific basis for research of soybean resistance and molecular breeding.