Mössbauer spectroscopy of frozen solutions as a stepwise control tool in preparation of biocompatible humic-stabilized feroxyhyte nanoparticles

Mossbauer spectroscopy of frozen aqueous solutions was demonstrated as an efficient stepwise control technique in the new one-pot synthesis of biocompatible feroxyhyte (δ ′-FeOOH) nanoparticles in situ stabilized by humic substances. Formation of ultradispersed Fe(OH)2 as an intermediate product and its interaction with humic substances were ascertained. The interaction of the surface of Fe(OH)2 with humic substances was considered as a starting point in the mechanism of in situ stabilization and further growth control of humic-stabilized feroxyhyte nanoparticles.