Comparison of Human-Human and Human-Mouse Hybridoma Systems for the Production of Alloreactive Human Monoclonal Antibodies

Compared with monoclonal antibodies of rodent origin, human monoclonal antibodies (HuMAbs) have the theoretical advantage of a better correlation with reaction patterns observed with alloantibodies, and in addition might have therapeutic application. A number of HuMAbs have been produced [3], but none to the major histocompatibility complex products have so far been described. The aim of our work was to evaluate the human-human and the human-mouse hybridoma systems for the production of alloreactive HuMAbs by performing parallel fusions between peripheral blood lymphocytes (PBLs) from women HLA-immunized by pregnancy and four 8-azaguanine-resistant mouse and human cell lines.