Microencapsulation Protects Immunoglobulin in Yolk (IgY) Specific against Helicobacter pylori Urease

Hens were intramuscularly (im) immunized on thighs by using urease (E.C. 3.5.1.5) from Helicobactor pylori as antigen. The specificity of IgY against urease of H. pylori increased gradually after initial immunization. The collected yolk was microencapsulated with 10% or 20% β-cyclodextrin (β-CD) and gum arabic by a spray-drier. Microencapsulation was effective in protecting the IgY activity against pepsin. Liposome prepared at the lecithin/ cholesterol ratio of 1/0.25 (mole/mole) displayed satisfactory encapsulation efficiency (69%) of IgY. Increase in cholesterol content in the liposomal structure exhibited a stronger protection effect of IgY against pepsin and acid. Des poules sont immunisees avec l'urease (EC 3.5.1.5) d'helicobacter pylori. Le jaune d'oeuf contenant les immunoglobulines Y (IgY) specifiques collecte peut etre microencapsulees en presence de β-cyclodextrine et de gomme arabique. L'encapsulation protege les IgY contre la degradation par la pepsine. Les IgY peuvent etre encapsules dans des liposomes (lecithine/cholesterol 1/0,25). L'augmentation de la teneur en cholesterol permet d'ameliorer la resistance a la pepsine