Back to Search
Start Over
Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids
- Source :
- Insect Molecular Biology. 28:1-22
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play essential roles in insect chemosensory recognition. Here, we identified nine OBPs and nine CSPs from the Myzus persicae transcriptome and genome. Genomic structure analysis showed that the number and length of the introns are much higher, and this appears to be a unique feature of aphid OBP genes. Three M. persicae OBP genes (OBP3/7/8) as well as CSP1/4/6, CSP2/9 and CSP5/8 are tandem arrayed in the genome. Phylogenetic analyses of five different aphid species suggest that aphid OBPs and CSPs are conserved in single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication. Motif pattern analysis revealed that aphid OBP and CSP motifs are highly conserved, and this could suggest the conserved functions of aphid OBPs and CSPs. Three OBPs (MperOBP6/7/10) are expressed antennae specifically, and five OBPs (MperOBP2/4/5/8/9) are expressed antennae enriched, consistent with their putative olfactory roles. M. persicae CSPs showed much broader expression profiles in nonsensory organs than OBPs. None of the nine MperCSPs were found to be antennae specific, but five of them (MperCSP1/2/4/5/6) showed higher expression levels in the legs than in other tissues. MperCSP10 mainly expressed in the antennae and legs. The broad and diverse expression patterns of M. persicae CSPs suggest their multifunctions in olfactory perception, development and other processes.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Aphid
biology
Phylogenetic tree
Odorant binding
media_common.quotation_subject
Chemosensory protein
Insect
biology.organism_classification
01 natural sciences
Genome
010602 entomology
03 medical and health sciences
030104 developmental biology
Insect Science
Odorant-binding protein
biology.protein
Molecular Biology
Gene
media_common
Subjects
Details
- ISSN :
- 09621075
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Insect Molecular Biology
- Accession number :
- edsair.doi...........0c549815c55f459768873d7e4f3a22b7