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Influence of najanalgesin from Naja naja on GLT-1 in spinal cord of rat in neuropathic pain
- Source :
- China Journal of Chinese Materia Medica.
- Publication Year :
- 2011
- Publisher :
- China Journal of Chinese Materia Medica, 2011.
-
Abstract
- OBJECTIVE To investigate the influence of najanalgesin on glutamate-glial transporter 1(GLT-1) in spinal cord of rats after L5 spinal nerve ligation and transection (SNL), and explore the spinal analgesic mechanism of najanalgesin. METHOD One hundred male SD rats were randomly divided into 6 groups: sham(A), SNL(B), SNL + najanalgesin(C), SNL + saline (D), SNL + najanalgesin + liposome (E), SNL + najanalgesin + liposome + GLT-1 As-ODNs(F) and treated with intrathecal injections of 10 p.L saline (A and D), 40 ng X kg(-1) najanalgesin (C, E and F), qd, respectively. Besides intrathecal administration of najanalgesin the rats were intrathecally injected with 10 microL of GLT-1 antisense oligodeoxynucleotides (As-ODNs) (F) and 10 micdroL of liposome(E) once daily on day 3. The L4-L6 segments of the spinal cord were isolated in 1, 4 and 7 d(A,B,C and D), 7 d(E and F) after surgery. The mRNA and protein of GLT-1 were determined. RESULT The SNL model has successfully been set up. Compared to sham group, the expression of GLT-1 mRNA and protein level in group B and D both increased firstly and decreased later, the expression of GLT-1 in group C was significantly increased and kept stable, which were also higher when compared to group D in day 7th. Compared to SNL + najanalgesin group, after intrathecal injection of GLT-1 As-ODNs the GLT-1, expression of GLT-1 in F group significantly decreased. While intrathecal administration of liposome had no significant effect on the spinal GLT-1 expression. CONCLUSION Najanalgesin could increase the mRNA and protein expression of GLT-1 in spinal cord, which may be one of its spinal mechanisms of analgesia.
- Subjects :
- Liposome
Messenger RNA
business.industry
medicine.medical_treatment
Analgesic
Pharmacology
medicine.disease
Spinal cord
medicine.anatomical_structure
Complementary and alternative medicine
Anesthesia
Neuropathic pain
Najanalgesin
Neuralgia
Medicine
Pharmacology (medical)
General Pharmacology, Toxicology and Pharmaceutics
business
Saline
Subjects
Details
- ISSN :
- 10015302
- Database :
- OpenAIRE
- Journal :
- China Journal of Chinese Materia Medica
- Accession number :
- edsair.doi...........0bed0aefc07262d0264fdb309d4d5722