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[Untitled]
- Source :
- Clinical and Experimental Medicine. 2:125-129
- Publication Year :
- 2002
- Publisher :
- Springer Science and Business Media LLC, 2002.
-
Abstract
- Chemokines are a group of structurally defined small proteins that act as chemoattractants for leukocytes and are involved in many different biological activities, including leukocyte activation for antimicrobial mechanisms. We studied the effect of the chemokines monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1α on nitric oxide release and parasitocidal ability of peripheral blood-derived human macrophages in vitro infected with Leishmania infantum, zymodeme MON1. In infected human macrophages, treatment with MCP-1 or MIP-1α significantly enhanced nitric oxide production and leishmanicidal ability, compared with untreated cells, to the same levels induced by interferon-γ. Both nitric oxide release and parasitocidal ability of macrophages were significantly reduced by addition of L-N Gmonomethylarginine (L-NMMA), which is a competitive inhibitor of the L-arginine nitric oxide pathway. These data suggest that MCP-1 and MIP-1α mediate macrophage activation for nitric oxide release and subsequent parasite clearance, and thus may play a role in the containment of Leishmania infection.
- Subjects :
- Chemokine
biology
Monocyte
General Medicine
biology.organism_classification
Leishmania
General Biochemistry, Genetics and Molecular Biology
Nitric oxide
Microbiology
chemistry.chemical_compound
medicine.anatomical_structure
chemistry
Nitric Oxide Pathway
biology.protein
medicine
Macrophage
Leishmania infantum
Macrophage inflammatory protein
Subjects
Details
- ISSN :
- 15918890
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Medicine
- Accession number :
- edsair.doi...........0b5de032ce162033ea39b631857a2a25