Dynamics of photosensitized singlet oxygen generation and photophysical characteristics of chlorin e 6 in photolon ointment

We have used luminescence and absorption spectroscopy to study the dynamics of photosensitized singlet oxygen generation and the photophysical characteristics of chlorin e 6 in Photolon ointment. We have shown that dimethylsulfoxide and polyethylene glycol 400, in concentrations comparable with their content in the ointment form of the drug Photolon, do not lead to appreciable changes in the spectral characteristics of chlorin e 6. Substantial changes in the photophysical characteristics of the photosensitizer are observed at significantly higher concentrations of the excipients in the ointment. We have shown that in buffer solution, chlorin e 6 forms a complex with polyethylene glycol 1500. For the first time, we have detected luminescence of singlet oxygen from the surface of biological tissue treated with Photolon ointment.