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Long Non-Coding RNA RP1130-1 Suppresses Cell Proliferation, Invasion and Migration Mediated by Downregulation of TGF-β
- Source :
- Journal of Biomaterials and Tissue Engineering. 9:822-828
- Publication Year :
- 2019
- Publisher :
- American Scientific Publishers, 2019.
-
Abstract
- Background: Hepatocellular carcinoma has low levels of long non-coding RNA (LncRNA) RP1130. However, the effects of LncRNA RP1130 in hepatocellular carcinoma still unknown. Materials and Methods: Expression of LncRNA RP1130-1 in HCC and cell lines were detected by real-time PCR. Cell proliferation was assessed by CCK-8. Wound-healing and Transwell assays were performed for HCC cell migration and invasion. Western blotting was carried out to evaluate cell cycle, migration and invasion associated proteins in HCC. Results: Expression levels of LncRNA RP1130-1 was dramatically lower in HCC tissues than in normal control. Similarly, LncRNA RP1130-1 was downregulated in HCC cell lines compared with LO2. The cellular experiments revealed that high expression of LncRNA RP1130-1 in HCC inhibited cell proliferation, migration and invasion. In addition, overexpression of LncRNA RP1130-1 inhibited the expression of transforming growth factor (TGF)-β, and TGF-β reversed the effects of LncRNA RP1130 in HCC cell lines. Conclusions: LncRNA RP1130 exerts anti-tumor effects mediated by inhibiting TGF-β. In summarize, our results indicate that LncRNA RP1130/TGFβ may be a potential therapeutic target for HCC.
Details
- ISSN :
- 21579083
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Biomaterials and Tissue Engineering
- Accession number :
- edsair.doi...........0a405604620228689a7b484a4d4ce4e4