Back to Search
Start Over
Ex Vivo Whole-Mount Imaging of Leukocyte Migration to the Bone Marrow
- Source :
- Methods in Molecular Biology ISBN: 9781071614242
- Publication Year :
- 2021
- Publisher :
- Springer US, 2021.
-
Abstract
- The bone marrow is the major hematopoietic organ, consisting of distinct microenvironmental niches for the production of hematopoietic cells. Advanced visualizing methods are required to define and better understand the interactions between stromal and hematopoietic cells. In this chapter, we describe an ex vivo whole-mount imaging technique of the bone marrow, which allows for a fast, high-quality, and three-dimensional visualization of different bone marrow components. We provide a guide for conducting adoptive transfer experiments of fluorescently labeled leukocytes and visualizing their location in the bone marrow with respect to the bone marrow vasculature. This method presents a quick, easy, and inexpensive approach to image the bone marrow in three dimensions.
- Subjects :
- 0301 basic medicine
Whole mount
Leukocyte migration
Pathology
medicine.medical_specialty
Adoptive cell transfer
Stromal cell
Hematopoietic organ
Biology
03 medical and health sciences
Haematopoiesis
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
030220 oncology & carcinogenesis
medicine
Bone marrow
Ex vivo
Subjects
Details
- ISBN :
- 978-1-07-161424-2
- ISBNs :
- 9781071614242
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071614242
- Accession number :
- edsair.doi...........09aa8df6ff1caf443f6231e3c8ffbd78