Oleoyl-CoA incorporation into triglycerides and phospholipids by chick enterocytes. Effect of cholesterol and cholestyramine feeding

Incorporation of 1[1- 14 Cloleoyl-CoA] into triglycerides was similar in villus and crypt cells from chick duodenum and lower than than in the upper and mid villus cells from jejunum. Cholesterol feeding produced a significant decrease of triglyceride synthesis in duodenum, while this specific activity increased in jejunum. Cholestyramine feeding did not affect triglyceride synthesis in duodenum and decreased that found in upper and mid villus cells from jejunum. Levels of oleoyl-CoA incorporation into phospholipids were higher than those observed in the incorporation into triglycerides. Cholesterol and cholestyramine feeding did not affect this activity. Incorporation of oleoyl-CoA into both triglycerides and phospholipids in enterocytes isolated from ileum was lower than that found in duodenum and jejunum, and did not change significantly by cholesterol or cholestyramine feeding.