In vitro propagation of Gossypium barbadense L. 'native cotton' of brown fiber

espanolGossypium barbadense L. ¨algodon nativo¨, especie originaria del Peru, de gran valor etnobotanico y de alto potencial industrial en el sector textil por presentar diferentes colores y tonalidades, y por ende no requerir de tintes; viene desapareciendo al igual que muchas de sus variedades, poseedoras de genes candidatos, que permitirian el mejoramiento de muchas variedades comerciales. La investigacion se centro en determinar la propagacion in vitro de G. barbadense “algodon nativo” de fibra marron. Las semillas procedieron del distrito de San Benito, Contumaza, Cajamarca-Peru. La fase experimental se desarrollo en el Laboratorio de Biotecnologia del Instituto de la Papa y Cultivos Andinos. Se empleo un diseno en bloques completamente al azar. Para el establecimiento in vitro se sembro nudos en medio MS (1962), suplementado con diferentes concentraciones de acido naftalenacetico (ANA) y 6 bencilaminopurina (BAP), los cuales constituyeron los tratamientos (T). Los resultados obtenidos fueron analizados estadisticamente con la prueba ANOVA y TUKEY, encontrandose diferencias estadisticamente significativas. Se concluye que para la propagacion in vitro de G. barbadense ¨algodon nativo¨ de fibra marron, las concentraciones 0,5 mg.L-1 de ANA y 2 mg.L-1 de BAP, son las mas adecuadas. EnglishGossypium barbadenseL. "native cotton", a species native to Peru, of great ethnobotanical value and high industrial potential in the textile sector by presenting different colors and shades, and therefore do not require dyes; has been disappearing like many of its varieties,possessing candidate genes that would allow the improvement of many commercial varieties. The research focused on determining the in vitropropagation of G. barbadense"native cotton"of brown fiber.The seeds came from the San Benito district, Contumaza, Cajamarca-Peru. The experimental phase was developed in the Biotechnology Laboratory of the Institute of Potato and Andean Crops. A completely randomized block design was used. For in vitro establishment, knots were seeded in MS medium (1962), supplementedwith different concentrations of naphthalenacetic acid (ANA) and 6 benzylaminopurine (BAP), which constituted the treatments (T). The results obtained were statistically analyzed with the ANOVA and TUKEY test, finding statistically significant differences. It is concluded that for the in vitropropagation of G. barbadense"nativecotton" of brown fiber, the concentrations 0.5 mg.L-1 of ANA and 2 mg.L-1 of BAP are the most adequate.