P3.236 Study of genital cancer aetiology: association of human papilomavirus (HPV) and merkel cell polyomavyrus (MCPYV)

Introduction The genital infection by the HPV is among the most frequent sexually transmitted diseases (STD) worldwide, and it may result in lesions that can lead to the carcinogenesis of the genital tract. However, other factors may be associated with the onset or progression of the tissue malignancy process, such as the MCPyV, which may present oncogenic profile in the epithelial tissue. This study aims to investigate the presence of MCPyV and HPV in malignant lesions of the male and female genital tract, in order to contribute to the elucidation of the role of these viruses in the cellular malignancy process and to the epidemiological knowledge regarding the prevalence of both viruses in neoplastic lesions. Methods This is a cross-sectional study evaluating the prevalence of HPV and MCPyV infection in samples of cervical carcinoma and penile cancer. To date, we have obtained 112 samples of penile carcinoma and 31 samples of cervical carcinoma. So, we aim to detect the presence of HPV DNA by the polymerase chain reaction (PCR) technique using the generic primers MY09/MY11; to genotype HPVs by specific PCR to the E6 gene; to detect and quantify DNA of the MCPyV by the Nested PCR technique and real-time PCR; to investigate the presence of truncation mutations in the major T antigen of MCPyV. Results Results are partial. To date, all the male samples were analysed. We verified the presence of HPV in 54 (48.2%) of these samples, in which the most prevalent type was the HPV16 (66%). The cervical carcinoma samples are still under analysis. Conclusion The collection of cervical neoplasia samples is still being performed. In 2015, our research group found a case of multiple infection by HPV, MCPyV and Epstein-Barr virus in a case of squamous cell carcinoma of the penis in Rio de Janeiro. This was the first report of the presence of MCPyV in this type of penile lesion. Thus, we look forward to find results that contribute to the presence of MCPyV in genital malignant lesions and to elucidate its role in the oncogenic pathway of malignant lesions.