Quantitative reaction of specific nitrogen fixation by protein antigens adsorbed on dermatol and paper

A specific addition of protein nitrogen was obtained in the agglutinate of dermatol particles, covered by antigen. Protein combined with azo-dye with bis-diazotized dianisidine gives stable sorption on the surface of the paper, cotton material or cotton wool. Such paper azo-antigen gives a pronounced specific addition of protein nitrogen which comes from the proteins of the antiserum. Analogous data were obtained with native paper antigens, secured by simple drying of the protein solution in which chromatographic paper is soaked. Reaction of specific fixation of the protein nitrogen on the surface of the paper antigen represents an easily performed new serological reaction in immunology. This reaction gives a numerical value of quantitative characteristics of the immunological antigen-antibody relation.