Effects of N-Butanol Fraction of Gongronema Latifolium Leave Extract on Some Liver Function and Histological Parameters in Ccl4-Induced Oxidative Damage in Wistar Albino Rats

Effects of n-butanol fraction of Gongronema latifolium leave extract on some liver function and histological parameters in CCl4-induced oxidative damage in Wistar albino rats were assessed. Fifty-four (54) Wistar albino rats were divided into treatment group and LD50 groups. Group A (normal control) was given feed and water, Group B (vehicle control) was injected with olive oil intraperitoneally, while the rest groups (C, D, E, F and G) were injected intraperitoneally with a single dose of CCl4 (148 mg/kg) as a 1:1 (v/v) solution in olive oil and all the animals were fasted for 36 hours. This was repeated once every week for a period of four (4) weeks. At the end of 28 days of treatment, liver marker enzymes studies showed that there was significant (p 0.05) difference of these liver marker enzymes and bilirubin levels between the normal control and induced treated groups. Antioxidant assay on the liver homogenate showed that there was significant (p 0.05) difference between the normal control and induced treated groups. These findings suggested that n-butanol fraction of methanolic leave extract of G. latifolium may have anti-hepatotoxic and antioxidative effects against CCl4-induced liver damage in rats.