Back to Search
Start Over
Salt-Gradient Approach for Regulating Capture-to-Translocation Dynamics of DNA with Nanochannel Sensors
- Source :
- ACS Sensors. 1:807-816
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- Understanding the physical mechanisms that govern the ion and fluidic transport in salt-concentration-based nanochannel/nanopore systems is essential for the potential applications in bioanalysis. One central challenge is to interpret the observed four-stage change from osmosis to the reverse one with increasing salt gradient. Here we provide a unified model that outlines the intriguing role of two competing factors, the exclusion- and diffusion-induced electrical potentials. We demonstrate theoretically a direction control of a hydrodynamic flow via the salt gradient. Based on this, we also propose a salt-gradient approach for regulating DNA motion in nanochannels that enables voltage-free single-molecule capture with a significantly low translocation speed. The present method would be used as a useful protocol to overcome the key hurdle of tailoring the capture-to-translocation dynamics of polynucleotides for nanopore sequencing.
- Subjects :
- Fluid Flow and Transfer Processes
Chemistry
Process Chemistry and Technology
Dynamics (mechanics)
Electrical potentials
Bioengineering
Nanotechnology
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
0104 chemical sciences
chemistry.chemical_compound
Nanopore
Present method
Fluidics
Nanopore sequencing
0210 nano-technology
Instrumentation
DNA
Hydrodynamic flow
Subjects
Details
- ISSN :
- 23793694
- Volume :
- 1
- Database :
- OpenAIRE
- Journal :
- ACS Sensors
- Accession number :
- edsair.doi...........06d1e14a46be54a6b012cc4f35afaca6