Amperometric glucose sensor based on enzyme-modified boron-doped diamond electrode by cross-linking method

A novel and robust amperometric enzyme electrode for the detection of glucose has been constructed by immobilizing glucose oxidase (GOD) on a boron-doped diamond (BDD) electrode with a cross-linking technique. Cyclic voltammograms were used to characterize the enzyme electrode. The response was evaluated with respect to the enzyme amount on the electrode and the concentration of BSA used in the cross-linking reagent. Linear calibration curve was obtained for glucose over the concentration range up to 35 mM in phosphate buffer, with the lowest experimental value measured being 0.5 mM.