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Action Spectra for Ultraviolet Photosensitized Killing of Mammalian Cells by Misonidazole and para-Nitroacetophenone
- Source :
- Radiation Research. 94:231
- Publication Year :
- 1983
- Publisher :
- JSTOR, 1983.
-
Abstract
- V-79 Chinese hamster fibroblasts in monolayer culture were exposed to ultraviolet radiation at 313, 334, 365, 380, and 405 nm in the presence of either misonidazole or para-nitroacetophenone, drugs which act as both photosensitizers and radiosensitizers of cell killing. Survival was measured by a colony-forming assay. The resulting action spectra for cell death photosensitized by the drugs (the reciprocals of the exposures required at each wavelength to reduce cell survival to a given level) closely match their absorption spectra over a range of three orders of magnitude. These results demonstrate that cells can be killed upon excitation of misonidazole or para-nitroacetophenone in the absence of any other types of energy deposition or biomolecular damage.
- Subjects :
- Programmed cell death
Misonidazole
Radiation
biology
Chemistry
Stereochemistry
Photodissociation
Biophysics
medicine.disease_cause
biology.organism_classification
Chinese hamster
Orders of magnitude (mass)
chemistry.chemical_compound
Cell killing
Cell culture
medicine
Radiology, Nuclear Medicine and imaging
Ultraviolet
Subjects
Details
- ISSN :
- 00337587
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- Radiation Research
- Accession number :
- edsair.doi...........052f1d9de8d4ae7535cde4487d86b4ab