Design and construction of synthetic cellulosome with three adaptor scaffoldins for cellulosic ethanol production from steam-exploded corn stover

In this study, a cellulosome-producing yeast reaction system with cellulase and hemicellulase activities is developed for synergistic catalysis of steam-exploded corn stover. Three adaptor scaffoldins with divergent specificities serve to contain an extensive type and amount of catalytic subunits. The maximum ethanol concentration of engineered yeast strain was 0.92 g/l corresponding to 53.44% of the theoretical yield based on grams of ethanol produced per gram of consumed total sugar under 30 °C with agitation at 90 rpm after 96 h. Here, our elaborated structural organization presents an approach designed toward genetic engineering of S. cerevisiae, a widespread, industrially important microorganism, for improved lignocellulolytic potential and advanced capability of consolidated bioprocessing.