Single-stranded conformation polymorphism analysis of Vitamin D receptor gene by capillary electrophoresis with laser-induced fluorescence detection

A highly sensitive single-stranded conformation polymorphism method for analysis of Vitamin D receptor (VDR) gene polymorphism was developed employing laser-induced fluorescence capillary electrophoresis (LIF-CE). LIF-CE was conducted utilizing a linear polyacrylamide solution as entangled polymer and acridine orange as a fluorescent dye of single-stranded DNA. Effect of acridine orange, size of PCR product and running temperature were investigated by LIF-CE in order to analyze the two polymorphisms of the allelic variation of the Bsm I and Taq I sites in intron 8 and exon 9, respectively, of the VDR gene. The developed method was simple, rapid (