Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam

Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to the Apocynaceae family. High similarity (98.2%) in the ITS1-5.8SrRNA-ITS2 gene sequence of CTW when compared on Genbank with J. tuyetanhiae and genetic distance difference (1.3%) - in the limit of difference of the same taxon considered on matK sequence shows that CTW is likely to belong to this species. Surface sterilization of ex vitro explants with HgCl2 (0.1%) for 3 min gave the highest sterilization efficiency compared to the remaining treatments with the induction explant rate of 93.33%. BA (0.5 mg l−1) is the most optimal for effective in vitro shoot multiplication in this species. Adding IBA to culture media at the appropriate concentration (0.5 mg l−1) increased rooting ability, shortened rooting time (13 days) and increased root length (6.53 mm). The ventilation culture system improves rooting efficiency, stimulates growth and induces secondary root formation—which is a suitable system for in vitro propagation of the Jasminanthes tuyetanhiae in this study. Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da Bia mountain of Phu Yen province. High similarity (98.2%) in the ITS1-5.8SrRNA-ITS2 gene sequence of CTW when compared on Genbank with Jaminanthes tuyetanhiae. In vitro propagation is an effective method for the conservation and development of the Jaminanthes tuyetanhiae plantlets on a commercial scale.