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Stepwise surface tailoring of carbon nanotubes with polyelectrolyte brushes and lipid layers to control their intracellular distribution and 'in vitro' toxicity
- Source :
- Soft Matter. 7:6883
- Publication Year :
- 2011
- Publisher :
- Royal Society of Chemistry (RSC), 2011.
-
Abstract
- Carbon Nanotubes (CNTs) have been functionalized with a layer of poly(sulfopropyl methacrylate) (PSPM) synthesized from silane initiators attached to the CNT walls. The PSPM brush was coated with a layer of poly(allyl amine hydrochloride) (PAH) and on top of the PAH layer lipid vesicles composed of 75% 1,2-dioleoyl-sn-glycero-3-phosphocholine and 25% 1,2-dioleoyl-sn-glycero-3-[phospho-L-serine] were assembled. The surface modification of the CNTs and lipid assembly were followed by Transmission Electron Microscopy (TEM) and ΞΆ-potential measurements. TEM and Confocal Raman Microscopy (CRM) were used to study the uptake and localization of the surface modified CNTs in HepG2 cells. PSPM modified CNTs were present in the cytoplasm. Proliferation studies based on the MTT assay were used to assess toxicity of the CNTs for the different modifications. Cell proliferation decreases for oxidized CNTs and polymer coated CNTs, having the lowest value for PSPM coated CNTs. The lipid coating on top of the polymers significantly reduces the toxic effect of CNTs.
- Subjects :
- 02 engineering and technology
Carbon nanotube
010402 general chemistry
Methacrylate
01 natural sciences
law.invention
symbols.namesake
chemistry.chemical_compound
law
Organic chemistry
chemistry.chemical_classification
Chemistry
General Chemistry
Polymer
021001 nanoscience & nanotechnology
Condensed Matter Physics
Silane
0104 chemical sciences
Chemical engineering
Transmission electron microscopy
symbols
Surface modification
lipids (amino acids, peptides, and proteins)
0210 nano-technology
Raman spectroscopy
Layer (electronics)
Subjects
Details
- ISSN :
- 17446848 and 1744683X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Soft Matter
- Accession number :
- edsair.doi...........047385d9358718d9f644259dbace5ce7