Molecular Dynamics of Rhodamine 6G Solutions as Revealed by the Computer Processing of Fluorescence Microscopy Images

In this article the image processing of workflow from raw camera frames is detailed. The results of the visualization and quantitative analysis of the images from the rhodamine 6 G solution obtained using total internal reflection fluorescence microscopy are presented. The main stages for image processing of fluorescent molecules are considered. The following actions are described: acquisition of raw data, noise reduction, segmentation of areas of interest by characteristic features, localization of potential fluorophores, postprocessing (assessment of area parameters). Spatial information (time dependence of relative movement of the molecule positions, the concentration distribution depending on the wavelength) is obtained. These results confirm the possibility of applying computer analysis to study the nature of the movement of molecules in a system, their interactions, and dynamic properties of various molecular systems.