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pH-Responsive Transferrin-pHlexi Particles Capable of Targeting Cells in Vitro

Authors :
Shenglin Yang
Georgina K. Such
Laura I. Selby
Adelene S. M. Wong
Rebecca Y. Hodgetts
Angus P. R. Johnston
Kristofer I. Cupic
Moore Z. Chen
Daniel Yuen
Ewa Czuba
Source :
ACS Macro Letters. 6:315-320
Publication Year :
2017
Publisher :
American Chemical Society (ACS), 2017.

Abstract

Targeting nanoparticles to specific cellular receptors has the potential to deliver therapeutic compounds to target sites while minimizing side effects. To this end, we have conjugated a targeting protein, holo-transferrin (holo-Tf), to pH-responsive polymers, poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-ran-poly(2-(diisopropylamino)ethyl methacrylate (PDEAEMA-r-PDPAEMA). These protein–polymer hybrid materials were observed to self-assemble when the pH is increased above the pKa of the polymer. We demonstrate that their response to pH could be tuned depending on the polymer constituent attached to holo-Tf. Importantly, the targeting behavior of these nanoparticles could be maximized by tuning the density of holo-Tf on the nanoparticle surface by the introduction of a (PDEAEMA-r-PDPAEMA)-b-poly(ethylene glycol) (PEG) copolymer.

Details

ISSN :
21611653
Volume :
6
Database :
OpenAIRE
Journal :
ACS Macro Letters
Accession number :
edsair.doi...........03fc38c48908dd0f5a2489d355ce1fcd
Full Text :
https://doi.org/10.1021/acsmacrolett.7b00044