Erratum to abstract 'Intracellular ice formation in mouse zygotes and early morulae vs. cooling rate and temperature–Experimental vs. theory' [Cryobiology 63(3) 2011 329]

It has been brought to notice that the name of the authors for the above mentioned abstract and the funding statement for this abstract has been missed during the typesetting. Hence, please find below the corrected versions of the abstract with all the details. The publisher apologizes for any inconvenience caused by the error. The correct abstract: 85. Intracellular ice formation in mouse zygotes and early morulae vs. cooling rate and temperature–Experimental vs. theory. ⁄Bo Jin, Peter Mazur, Fundamental and Applied Cryobiology Group, Department of Biochemistry and Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996-0840, USA. In 1972, Whittingham, Leibo, and Mazur reported successful cryopreservation of 8-cell mouse embryos. They found that plots of their survival vs. cooling rate (CR) take the form of an inverted U. They also reported on the survival of 2-cell embryos and blastocysts as function of CR. These two stages also yielded an inverted U with a somewhat similar shape. They hypothesized that the drop in survival above CR of 1 C/min was due to intracellular ice formation (IIF). Subsequent papers showed that hypothesis to be correct for 8-cell embryos, but it has never been demonstrated for zygotes and morulae. That was the purpose of the work reported here. In this study, mature female mice of the ICR strain were induced to superovultate, mated, and collected at either zygote or early morula stages. Embryos suspended in 1 M EG in PBS containing 10 mg/ LSnomax for 15 min, then transferred in sample holder to Linkam