Immobilization of Enzymes

Immobilized enzymes are provided in which the enzyme is covalently linked by a disulphide group-containing bridge to an inorganic or organic support carrier; the carrier material comprising the support carrier bearing mercaptan groups is readily regenerated from spent immobilized enzyme by reduction under mild conditions of the disulphide group and fresh enzyme can then be immobilized without waste of the support carrier the method of immobilizing the enzymes is applicable to a wide variety of enzymes, since it does not require any specific functional groups; the method is especially useful for immobilizing expensive enzymes for example, glucose isomerase.