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Production of Germ-Line Chimeras in Zebrafish
- Source :
- Methods in Molecular Biology ISBN: 9781493990085
- Publication Year :
- 2019
- Publisher :
- Springer New York, 2019.
-
Abstract
- The induction of germ-line chimerism in fish is a strategy for the reproduction of endangered or genetically valuable fish species. Chimeras can be created by transplanting a single primordial germ cell or multiple blastomeres from a donor into a sterile host embryo. When the host reaches sexual maturity, it will produce donor-originating gametes throughout its reproductive life span. This technique provides unique experimental conditions for basic biology research in model fish species like zebrafish. The success of cell transplantation relies on the effective sterilization of host embryos, the correct identification of developing germ cells, and the synchronization of migratory cues between the host and the transplanted cells. Developments in non-transgenic methods of germ cell ablation and identification have made germ cell transplantation more applicable to use in conservation and aquaculture. In this chapter, we provide a protocol for germ cell labeling by injection of chimeric RNA or FITC-dextran, the sterilization of host embryos using an antisense morpholino oligonucleotide, and two methods for producing germ-line chimeras in zebrafish: single primordial germ cell transplant and blastomere transplant.
- Subjects :
- 0301 basic medicine
Morpholino
Embryo
Blastomere
Biology
biology.organism_classification
Germline
Cell biology
Transplantation
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Chimeric RNA
medicine
Zebrafish
030217 neurology & neurosurgery
Germ cell
Subjects
Details
- ISBN :
- 978-1-4939-9008-5
- ISBNs :
- 9781493990085
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781493990085
- Accession number :
- edsair.doi...........0215fc7c8eae104b416922e23ae22a8e