Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration Use of X-ray microprobe to diagnose bone tissue demineralization after caffeine administration

Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has beenshown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was toassess the influence of caffeine on the development of bone tissue, with particular reference to elementalbone composition using an X-ray microprobe. The research was conducted on rats. The fertilized femaleswere randomly divided into an experimental and a control group. The experimental group wasgiven caffeine orally in 30 mg/day doses from the 8th to the 21st day of pregnancy, while the control groupwas given water. The fetuses were used to assess the growth and mineralization of the skeleton. On thebasis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted.For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimentalgroup, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of thedevelopment of variants in the experimental group was statistically higher. In the experimental group,a significant decrease in the calcium level, as well as an increase in the potassium level, was observed.The X-ray microprobe’s undoubted advantage is that is offers a quick qualitative and quantitative analysisof the elemental composition of the examined samples. Employing this new technique may furnish uswith new capabilities when investigating the essence of the pathology process.Caffeine is a methylxanthine which permeates the placenta. In studies on animals, it has beenshown to produce teratogenic and embryotoxic effects in large doses. The objective of this study was toassess the influence of caffeine on the development of bone tissue, with particular reference to elementalbone composition using an X-ray microprobe. The research was conducted on rats. The fertilized femaleswere randomly divided into an experimental and a control group. The experimental group wasgiven caffeine orally in 30 mg/day doses from the 8th to the 21st day of pregnancy, while the control groupwas given water. The fetuses were used to assess the growth and mineralization of the skeleton. On thebasis of double dyeing, a qualitative analysis of the bone morphology and mineralization was conducted.For calcium and potassium analysis, an X-ray microprobe was used. In 67 fetuses from the experimentalgroup, changes in skeleton staining with the alcian-alizarin method were noticed. The frequency of thedevelopment of variants in the experimental group was statistically higher. In the experimental group,a significant decrease in the calcium level, as well as an increase in the potassium level, was observed.The X-ray microprobe’s undoubted advantage is that is offers a quick qualitative and quantitative analysisof the elemental composition of the examined samples. Employing this new technique may furnish uswith new capabilities when investigating the essence of the pathology process.