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Induction of Probiotic Strain Enterococcus faecium EF1 on the Production of Cytokines, Superoxide Anion and Prostaglandin E2 in a Macrophage Cell Line
- Source :
- Pakistan Veterinary Journal, Vol 32, Iss 4, Pp 530-534 (2012)
- Publication Year :
- 2012
- Publisher :
- University of Agriculture, Faisalabad, 2012.
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Abstract
- Immunological effects of probiotic strain Enterococcus faecium EF1 was evaluated through the production of cytokines, superoxide anion (O2.-) and prostaglandin E2 (PGE2) in murine macrophage cell line RAW 264.7. In addition, the responses were contrasted with a pathogenic coliform, Escherichia coli strain K88. After 12-h co-incubation of RAW 264.7 with E. faecium EF1 or E. coli K88, the culture supernatants were collected for further analysis of cytokines (TNF-α, IFN-γ, IL-1β, IL-6, IL-12, IL-8 and IL-10) and inflammatory mediators including superoxide anion (O2.-) and PGE2. The results showed that, E. faecium EF1 induced an increased (P
Details
- Language :
- English
- ISSN :
- 02538318
- Volume :
- 32
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Pakistan Veterinary Journal
- Accession number :
- edsair.doajarticles..b9bd88f60796ba5b0d356fb006e35a99