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Effects of insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) on the proliferation of rabbit articular chondrocytes embedded in microfluidic chip
- Source :
- Medical Journal of Chinese People's Liberation Army, Vol 38, Iss 6, Pp 476-480 (2013)
- Publication Year :
- 2013
- Publisher :
- Editorial Board of Medical Journal of Chinese People's Liberation Army, 2013.
-
Abstract
- Objective To investigate the effects of insulin-like growth factor-1 (IGF-1) and basic fibroblast growth factor (bFGF) on the proliferation of chondrocytes in a 3-dimensional (3D) culture environment of microfluidic chip. Method Rabbit articular chondrocytes embedded in microfluidic chip and 96-well plate were cultured with solitary and component concentrations of IGF-1 and bFGF for 2 weeks, and then the proliferation rates of chondrocytes were calculated and compared. Results The maximal proliferation effect of IGF-1 was found when the concentration reached 57.14ng/ml (up to 2.38-fold), and of bFGF at the concentration of 5.72ng/ml (up to 3.85-fold), while the maximal proliferation effect of combination of IGF-1+bFGF was found at the concentration of 85.71ng/ml IGF-1 plus 1.43ng/ml bFGF (up to 4.76-fold). There was no significant difference between the proliferation rate of chondrocytes cultured in microfluidic chip and 96-well plate. Conclusions A combination of IGF-1 plus bFGF in certain concentration may synergistically increase the proliferation of chondrocytes. Microfluidic chip could be used for efficient cartilage tissue engineering.
Details
- Language :
- Chinese
- ISSN :
- 05777402
- Volume :
- 38
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Medical Journal of Chinese People's Liberation Army
- Accession number :
- edsair.doajarticles..9ede6038440c5b65c750762299371cd6