Identification and expression analysis of glycolate oxidase gene family in spinach

The spinach (Spinacia oleracea L.) glycolate oxidase (GLO) gene family members were identified from the whole genome of spinach,and their physical and chemical properties,subcellular localization,gene structure,conserved motifs,homology and gene expression were analyzed.Results showed that there are five SoGLOs proteins in spinach.The genetic relationship between spinach's GLO protein and those of Beta vulgaris L. protein was relatively close through phylogenetic tree analysis.Gene structure analysis revealed that the family gene consisted of 9 to 11 exons.Real-time quantitative polymerase chain reaction (qRT-PCR) showed that nitrate nitrogen could only induce SoGLOs expression in a short period of time,while ammonium nitrogen could continue to inhibit the expression of SoGLOs,thus affecting the content of spinach oxalic acid.After stress treatment,the expression of SoGLOs changed significantly.SoGLO1,SoGLO3 and SoGLO5 responded most to salt stress.SoGLOs may play a role in the salt tolerance,high temperature tolerance,cold tolerance,drought resistance and antioxidant process of spinach.Spraying of phytohormones generally increases the expression of SoGLOs in a short period of time,which may cause rapid accumulation of oxalic acid in spinach.