Hematological and biochemical indexes in blood of HBV transgenic mice

Objective To study the effects of gene integration of HBV on the biochemical and hematological indices in blood of transgenic mice.Methods The venous blood was collected from orbital venous plexus of 28 normal mice born in the same brood(HBsAg negative) and 50 HBV transgenic mice(HBsAg positive),6-8 weeks in age.The blood routine examination was performed,including white blood cells(WBC),red blood cells(RBC),hemoglobin(Hb),platelets(PLT),lymphocyte percentage(L%),intermediate cell percentage(M%),the percentage of leaf cells(G%) and blood biochemical parameters including glucose(GLU),urea nitrogen(BUN),creatinine(CREA),total protein(ALB),albumin(ALB),total bilirubin(TBIL),alanine aminotransferase(ALT),aspartate aminotransferase(AST),total cholesterol(CHOL) and triglyceride(TG).The differential indexes were analyzed in HBsAg positive and negative mice,also the mice of different sex.Results Significant differences were found between the transgenic and normal mice in blood GLU,BUN,CREA,RBCs,Hb and PLT(P < 0.05),while no significant differences were found in other indices.No difference was found in the above 6 indices between the different sex of normal mice,while there was significant difference in blood GLU between males and females in transgenic mice,and it was higher in males than in females(P < 0.05).The blood GLU,CREA and BUN were higher in transgenic male mice than in normal male mice,and the contents of RBCs,Hb and PLT were higher in transgenic female mice than in normal female mice(P < 0.05).Conclusion HBV DNA may throw some influences on blood biochemical and hematological indexes of HBV transgenic mice,and it may provide some valuable references to the study of HBV pathogenesis in HBV animal models.