Over-expression of ROBO4 reduces permeability of human renal glomerular endothelial cells in high glucose medium

Objective To investigate the effect of over-expression of ROBO4 on permeability of human renal glomerular endothelial cells (HRGECs) in high glucose medium. Methods HRGECs infected with recombinant lentiviral vector ROBO4 were cultured in high glucose or low glucose medium in vitro. The protein levels of ROBO4 and ARF6 in each group were detected by Western blotting. The endothelial permeability was measured by the efflux of fluorescein isothiocyanate-dextran (FITC-Dextran) permeated through the monolayer endothelial cells using Transwell cell model system. The cell viability after lentivirus transfection was measured by CCK8 assay. Results The transfection rate of lentiviruses in HRGECs reached 80% 72h after, and obvious over-expression of ROBO4 protein was in transformed cells compared with the empty vector group (P