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PDA-mediated Mild Photothermal Therapy Combined with Autophagy Inhibitors Kill Breast Cancer Cells

Authors :
LIU Yawen
LU Jiahui
NI Chen
HUANG Jie
HUANG Tianhao
SHEN Nan
DONG Yulin
SHI Meilin
HU Junfeng
Source :
Zhongliu Fangzhi Yanjiu, Vol 48, Iss 7, Pp 659-666 (2021)
Publication Year :
2021
Publisher :
Magazine House of Cancer Research on Prevention and Treatment, 2021.

Abstract

Objective To explore whether inhibiting autophagy can enhance the sensitivity of photothermal treatment under mild photothermal conditions. Methods CQ@PLGA@PDA NPs were prepared by an improved double emulsification method and a PDA-based surface modification method. After basic characterization, CCK-8 method was used to detect the cytotoxicity of nanoparticles; the near-infrared laser irradiation nanoparticle solution was used to detect the heating effect; CCK-8 method and live-dead cell staining were used to detect the killing effect of tumor cells; Western blot was used to detect the expression of autophagy-related proteins. Results The CQ@PLGA@PDA NPs were successfully prepared, with a particle size of 253.10±2.39 nm, a zeta potential of -22.57±0.80 mV, uniform particle size and good dispersion. The temperature of nanoparticle solution increased to 45℃ after the near-infrared laser irradiation for 10 min. CQ@PLGA@PDA NPs had no obvious toxicity to cells. The survival rates of breast cancer cell MDA-MB-231 and mouse embryonic fibroblast NIH-3T3 cell were above 95%. The inhibition of autophagy under mild photothermal conditions could improve the sensitivity of photothermal therapy. Conclusion The prepared CQ@PLGA@PDA NPs have good photothermal performance and high biological safety; by inhibiting autophagy, they can effectively kill tumor cells under mild photothermal conditions(< 50℃).

Details

Language :
Chinese
ISSN :
10008578
Volume :
48
Issue :
7
Database :
OpenAIRE
Journal :
Zhongliu Fangzhi Yanjiu
Accession number :
edsair.doajarticles..0187cf7856df293b943fa0904da1617c