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Placental and fetal steroidogenesis
- Source :
- Methods in Molecular Biology (Clifton then Totova), Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2009, 550, pp.127-36. 〈10.1007/978-1-60327-009-0_7〉
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- International audience; Steroid hormones are essential for maintenance of pregnancy and fetal development. The expression and catalytic activity of the key steroidogenic enzymes involved in the production of progesterone and estrogens increase during pregnancy, and there is an intricate communication between the mother, the placenta, and the fetus in order to maintain a balanced supply of the steroid hormones essential for embryogenesis. This chapter describes methods for the measurement of the expression and catalytic activity of three key cytochrome P450 (CYP) enzymes involved in the production of progesterone and estrogens, aromatase (CYP19), steroid 17-hydroxylase/17,20-lyase (CYP17), and cholesterol side-chain cleavage (CYP11A).
- Subjects :
- MESH : Pregnancy
MESH : RNA, Messenger
MESH : Tumor Cells, Cultured
MESH : Placenta
MESH : Humans
[ SDV.TOX ] Life Sciences [q-bio]/Toxicology
MESH : Aromatase
MESH : Reverse Transcriptase Polymerase Chain Reaction
MESH : Base Sequence
MESH : DNA Primers
MESH : Female
MESH : Fetus
MESH : Steroids
Subjects
Details
- Language :
- English
- ISSN :
- 10643745 and 19406029
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology (Clifton then Totova), Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2009, 550, pp.127-36. 〈10.1007/978-1-60327-009-0_7〉
- Accession number :
- edsair.dedup.wf.001..ffb5cedca3fb65d901323a9f768a614e