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Cell wall lipopeptides of Mycobacterium avium: new insights from genomics analysis

Cell wall lipopeptides of Mycobacterium avium: new insights from genomics analysis

Authors :
Bannantine, John
Etienne, Gilles
Laval, Françoise
Lemassu, Anne
Daffé, Mamadou
Bayles, Darrell O.
Ganneau, Christelle
BRANGER, Maxime
Cochard, Thierry
Stabel, Judith R.
Bay, Sylvie
Biet, Franck
National Animal Disease Center
USDA
Institut de pharmacologie et de biologie structurale (IPBS)
Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Université Fédérale Toulouse Midi-Pyrénées
Institut Pasteur [Paris]
Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids
Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Infectiologie et Santé Publique (UMR ISP)
Institut National de la Recherche Agronomique (INRA)-Université de Tours
Société Française de Microbiologie (SFM). FRA.
Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)
Source :
5. Conférence du MycoClub ( Mycobactéries 2017), 5. Conférence du MycoClub ( Mycobactéries 2017), May 2017, Montigny le Bretonneux, France. 2017
Publication Year :
2017
Publisher :
HAL CCSD, 2017.

Abstract

International audience; Mycobacteria have a complex cell wall structure that includes many lipids which are often species specific. Besides giving a phenotypic signature these lipids are often involved in infectious processes of pathogenic mycobacteria by interfering with the host immune system. The biosynthesis pathway of the simplest glycopeptidolipids (GPLs) is relatively well understood and involves more than fifteen genes. Even though M. avium subsp. paratuberculosis (Map) produces a lipopeptide rather than GPL, its genome contains nevertheless a locus highly similar to the GPL biosynthetic pathway of M. avium subsp. avium (Mav). We showed that the module composition of the non-ribosomal protein synthase (Nrp) of Map, the enzyme involved in the synthesis of the peptidyl moiety, is dramatically different from that of other GPL producers such as M. smegmatis (Ms) and Mav. While Map isolates do not produce GPLs, they do produce lipopeptides without the carbohydrate moiety. However, the picture is not as clear regarding the diversity of lipopeptides produced among two lineages classified as type I/III or S-type (ovine) and type II or C-type (bovine) Map strains that have emerged from the common ancestor, M. avium subsp. hominissuis. The S-type isolates are readily distinguishable from C-type isolates based on genome studies and readily discriminated by genotyping methods. In addition to the genotypic distinctions between S- and C-type strains, phenotypic differences have been documented. To provide a genomic basis for the synthesis of the diversity of lipopeptides in Map, its recently published genome sequence was explored using in silico methods and completed by biochemical investigations. Interestingly we discovered a change in the chemical structure of the lipopeptide of the S strains. These findings add new phenotypic evidence that contribute to separate the S type to the C type. Furthermore deciphering the biosynthesis pathway of cell wall lipopeptides should contribute to better understand the determinants of the adaptation of a pathogen to a specific host but also the factors favoring transmission to a new host.

Details

Language :
English
Database :
OpenAIRE
Journal :
5. Conférence du MycoClub ( Mycobactéries 2017), 5. Conférence du MycoClub ( Mycobactéries 2017), May 2017, Montigny le Bretonneux, France. 2017
Accession number :
edsair.dedup.wf.001..ec135feb6bef145c2a0832b1efe5507d