Allele specific expression as an indication of ploidy in pig IPECJ2 and chicken SL-29 cell lines

Authors :: de Vos, J.
Crooijmans, R.P.M.A.
Derks, M.F.L.
Kloet, S.L.
Groenen, M.A.M.
Madsen, O.
Source :: Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP). Wageningen: Wageningen Academic Publishers, Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Publication Year :: 2022
Publisher :: Wageningen Academic Publishers, 2022.
Abstract: Cell lines are useful for investigating traits of interest e.g. intestinal absorption, feed efficiency and immunity in farm animals. We earlier investigated an intestinal cell line in pig and a fibroblast cell line in chicken and found chromosomal abnormalities by whole genome sequence (WGS) data analysis. Results from RNAseq allele-specific expression analysis (ASE) in 4 cell lines showed aneuploidy in some chromosomes. In this paper we show that RNA-seq can be used to detect whole/partial chromosomal abnormalities based on ASE analysis.

Subjects :: WIAS
Life Science
Fokkerij en Genomica
Animal Breeding and Genomics

Language :: English
ISBN :: 978-90-8686-940-4
ISBNs :: 9789086869404
Database :: OpenAIRE
Journal :: Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP). Wageningen: Wageningen Academic Publishers, Proceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Accession number :: edsair.dedup.wf.001..c5436daca6321ef92c58c3ad5998bd5e

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