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Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells
- Source :
- International journal of obesity (2005), vol 31, iss 1, Vangipuram, SD; Yu, M; Tian, J; Stanhope, KL; Pasarica, M; Havel, PJ; et al.(2007). Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells.. International journal of obesity (2005), 31(1), 87-96. doi: 10.1038/sj.ijo.0803366. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/4j27h679
- Publication Year :
- 2007
- Publisher :
- eScholarship, University of California, 2007.
-
Abstract
- ObjectiveHuman adenovirus Ad-36 causes adiposity in animal models and enhances differentiation and lipid accumulation in human and 3T3-L1 preadipocytes, which may, in part, explain the adipogenic effect of Ad-36. We determined the consequences of Ad-36 infection on leptin and glucose metabolism in fat cells.Design3T3-L1 preadipocytes were used to determine the effect of infection by human adenoviruses Ad-36, Ad-2, Ad-9 and Ad-37 on leptin secretion and lipid accumulation. Rat primary adipocytes were used to determine the effect of Ad-36 infection on leptin secretion and glucose uptake in vitro. Furthermore, the effect of Ad-36 on expressions of leptin and selected genes of de novo lipogenesis pathway of visceral adipose tissue were compared ex vivo, between Ad-36 infected and uninfected control rats.ResultsAd-36 suppressed the expression of leptin mRNA in 3T3-L1 cells by approximately 58 and 52% on days 3 and 5 post-infection, respectively. Leptin release normalized to cellular lipid content was 51% lower (P
- Subjects :
- Leptin
Male
Cells
Messenger
Wistar
Gene Expression
Intra-Abdominal Fat
Adenovirus Infections
Medical and Health Sciences
Education
Adenovirus Infections, Human
Mice
Endocrinology & Metabolism
infectobesity
3T3-L1 Cells
Adipocytes
Animals
Humans
RNA, Messenger
Obesity
Rats, Wistar
glucose
Cells, Cultured
3T3-L1
adiposity
lactate
Cultured
Adipogenesis
Lipid Metabolism
Rats
Fatty Acid Synthetase Complex
Ad-36
RNA
Fatty Acid Synthases
Human
Acetyl-CoA Carboxylase
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- International journal of obesity (2005), vol 31, iss 1, Vangipuram, SD; Yu, M; Tian, J; Stanhope, KL; Pasarica, M; Havel, PJ; et al.(2007). Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells.. International journal of obesity (2005), 31(1), 87-96. doi: 10.1038/sj.ijo.0803366. UC Davis: Retrieved from: http://www.escholarship.org/uc/item/4j27h679
- Accession number :
- edsair.dedup.wf.001..bc909d72f39a51b6dea8e7f380c43734