Changes in chromatin state derived of sperm capacitation in red deer spermatozoa

Trabajo presentado a la 20th Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR) and the 13th Conference of the Spanish Association for Animal Reproduction (AERA), celebradas en Lisboa (Portugal) del 27 al 29 de octubre de 2016.
In this study it was evaluated the state of chromatin after a capacitation time in red deer spermatozoa. Thawed semen from three red deer was used after selection in 45% Percoll. Spermatozoa were capacitated with a synthetic oviductal fluid (SOF) with 20% estrus sheep serum (ESS), and the high DNA stability (HDS) was evaluated at different incubation times (1, 5, 15, 30, 45, 60, 120 min and 24 h) in the capacitation medium. In addition, samples of non-capacitated spermatozoa (incubated with SOF without ESS) were evaluated at 0, 120 min and 24 h. A heterologous vitro fertilization trial, oocyte from sheep and spermatozoa from deer, was performed with capacitated sperm samples for 5, 15, 30 and 60 m in and non-capacitated sperm samples at0 m in. Results showed that HDS increased with the incubation time with the highest values at 15 (6.48), 30 (6.14) and 45 (6.06) min. of incubation in relation to non-capacitated sperm samples initially evaluated. However, the highest percentage of cleaved embryos was register for capacitated spermatozoa for 5 (19.5%), 15 (19.88%) and 30 (18.19%) min., with lower values for non-capacitated samples (5.6%) or 60 m in capacited (8.36%). More studies are needed to know the relation of the changes in chromatin state and the fertilization ability after capacitation time.