Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA

Authors :: Kristensen, Torsten
Schousboe, Inger
Boel, Espen
Mulvihill , Eileen M
Hansen, Rita Rosendahl
Møller, Karin Bach
Møller, Niels Peter Hundahl
Sottrup-Jensen, Lars
Source :: Kristensen, T, Schousboe, I, Boel, E, Mulvihill, E M, Hansen, R R, Møller, K B, Møller, N P H & Sottrup-Jensen, L 1991, ' Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA. ', FEBS Letters, vol. 289, no. 2, pp. 183-186 .
Publication Year :: 1991
Abstract: Human β2-glycoprotein (β2gpI) cDNA was isolated from a liver cDNA library and sequenced. The cDNA encoded a 19-residue hydrophobic signal peptide followed by the mature β2gpI of 326 amino acid residues. In liver and in the hepatoma cell line HepG2 there are two mRNA species of about 1.4 and 4.3 kb, respectively, hybridizing specifically with the β2gpI cDNA. Upon isoelectric focusing, recombinant β2gpI obtained from expression of β2gpI cDNA in baby hamster kidney cells showed the same pattern of bands as β2gpI isolated from plasma, and at least 5 polypeptides were visible

Language :: English
Database :: OpenAIRE
Journal :: Kristensen, T, Schousboe, I, Boel, E, Mulvihill, E M, Hansen, R R, Møller, K B, Møller, N P H & Sottrup-Jensen, L 1991, ' Molecular cloning and mammalian expression of human beta 2-glycoprotein I cDNA. ', FEBS Letters, vol. 289, no. 2, pp. 183-186 .
Accession number :: edsair.dedup.wf.001..b231e3262d8c4e9bb36d9e7628a943c7