Classifying bacterial isolates from hypogean environments: application of a novel fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature

Classification of bacterial isolates is essential for the development of the microbiology of art objects. However, obtaining all the information needed for describing a new species or taxon is often difficult. G+C mol% content in microorganisms is one of the recommended characteristics for the standard description of bacterial species. In this study we present a novel fluorimetric method to estimate the G+C mol% content in microorganisms.
J.M. González and L. Laiz were supported by the Ramón y Cajal program of the Spanish Ministry of Science and Technology and the CSIC-13P programm, respectively. This work was carried out within the framework of the EC projects: CATS (EVK4-CT-2000-00028), COALITION (EVK4-CT-1999-20001) and the Spanish project BTE2002-04492-C02-01).