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Bulk staining of smears: no demonstrated risk of bacilli transfer from a positive to a negative smear
- Source :
- International journal of tuberculosis and lung disease
- Publication Year :
- 2008
- Publisher :
- International Union Against Tuberculosis And Lung Disease, 2008.
-
Abstract
- Despite a theoretical risk of transfer of bacilli from a positive to a negative smear, bulk staining is routinely performed in many laboratories. To assess this risk in our laboratory, two smears were made from each sputum specimen and stained with auramine: one smear was stained on a rack and the second using the bulk method. Smears were read blind using a fluorescence microscope. A total of 811 sputum specimens were analysed. No acid-fast bacilli transfer was observed even when staining solution jars had not been renewed for 3 days. Bulk staining is rapid and cheap, and could be used in laboratories with a high workload in low-resource settings.
Details
- Language :
- English
- ISSN :
- 10273719
- Database :
- OpenAIRE
- Journal :
- International journal of tuberculosis and lung disease
- Accession number :
- edsair.dedup.wf.001..9e45838d5b216d2c70b713eca72f6d13