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Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering
- Source :
- Advanced healthcare materials, vol 5, iss 1
- Publication Year :
- 2016
- Publisher :
- eScholarship, University of California, 2016.
-
Abstract
- Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In this study, a photocrosslinkable gelatin (i.e., gelatin methacrylamide (GelMA)) with tunable mechanical, degradation, and biological properties is used to engineer the epidermis for skin tissue engineering applications. The results reveal that the mechanical and degradation properties of the developed hydrogels can be readily modified by varying the hydrogel concentration, with elastic and compressive moduli tuned from a few kPa to a few hundred kPa, and the degradation times varied from a few days to several months. Additionally, hydrogels of all concentrations displayed excellent cell viability (>90%) with increasing cell adhesion and proliferation corresponding to increases in hydrogel concentrations. Furthermore, the hydrogels are found to support keratinocyte growth, differentiation, and stratification into a reconstructed multilayered epidermis with adequate barrier functions. The robust and tunable properties of GelMA hydrogels suggest that the keratinocyte laden hydrogels can be used as epidermal substitutes, wound dressings, or substrates to construct various in vitro skin models.
- Subjects :
- keratinocytes
Compressive Strength
Light
Cell Survival
Sus scrofa
Medical Biotechnology
Biomedical Engineering
photocrosslinkable gelatin
Bioengineering
macromolecular substances
mechanical properties
Regenerative Medicine
complex mixtures
Medicinal and Biomolecular Chemistry
Elastic Modulus
epidermis
Cell Adhesion
Electric Impedance
Animals
Humans
Cell Proliferation
degradation
Skin
Acrylamides
integumentary system
Tissue Engineering
Tissue Scaffolds
technology, industry, and agriculture
Hydrogel
Cross-Linking Reagents
Polyethylene Glycol Dimethacrylate
Epidermal Cells
Gelatin
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Advanced healthcare materials, vol 5, iss 1
- Accession number :
- edsair.dedup.wf.001..9cf4f79a289cf0042306e067c0abf9b8