Expression of apoptotic and proliferative factors in the gastric mucosa of patients with systemic sclerosis

U istraživanju „Izražaj apoptotskih i proliferacijskih čimbenika u želučanoj sluznici oboljelih od sistemske skleroze“ histološki su analizirane razlike u proliferaciji, apoptozi i profibrotičkim čimbenicima u uzorcima želučane sluznice oboljelih od sistemske skleroze (SSc) i kontrolne skupine. U istraživanju je sudjelovao 15 bolesnika sa SSc i 5 kontrolnih ispitanika bez SSc. Bolesnici su sistematizirani s obzirom na oblik i težinu kliničke slike sistemske skleroze. Pet bolesnika je klasificirano u skupinu s ograničenom kožnom SSc (lcSSc) i blagom bolešću, pet s umjerenom difuznom i pet s teškom difuznom kožnom SSc (dcSSc). Uzorci tkiva su imunohistokemijski obojeni protutijelom Ki-67 (proliferacija), kaspazom-3 (apoptoza), sindekanom-1 (epitelni biljeg), CTGF (profibrotički biljeg) i α-SMA (biljeg miofibroblasta). Istovremena prisutnost (kolokalizacija) Ki-67 pozitivnih stanica s biljegom za miofibroblaste (α-SMA) određena je upotrebom dvostruke imunofluorescentne metode. Postotak reaktivnih stanica analiziran je primjenom Kruskal-Wallis testa i Dunn-ovog post hoc testa, a P
In the study "Expression of apoptotic and proliferative factors in the gastric mucosa of patients with systemic sclerosis" differences in proliferation, apoptosis and profibrotic factors were histologically analyzed in samples of gastric mucosa in patients with systemic sclerosis (SSc) and control group. The study included 15 patients with SSc and 5 control subjects without SSc. Patients were systematized with respect to the type and severity of systemic sclerosis. Five patients were classified into the group with limited cutaneous SSc (lcSSc) and mild disease, five with moderate diffuse, and five with severe diffuse cutaneous SSc (dcSSc). Tissue samples were immunohistochemically stained with Ki-67 antibody (proliferation), caspase-3 (apoptosis), syndecan-1 (epithelial marker), CTGF (profibrotic marker), and α-SMA (myofibroblast marker). The simultaneous presence (colocalization) of Ki-67 positive cells with α-SMA was determined using double immunofluorescence method. The percentage of reactive cells was analyzed by a Kruskal-Wallis test and the Dunn post hoc test. P