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A simple dilute-and-shoot approach for the determination of ultra-trace levels of arsenic in biological fluids via ICP-MS using CH3F/He as a reaction gas

Authors :
Flórez, M.R.
García-Ruiz, E.
Bolea-Fernández, E.
Vanhaecke, F.
Resano, M.
Source :
Zaguán. Repositorio Digital de la Universidad de Zaragoza, instname
Publication Year :
2017

Abstract

The performance of a mixture of CH3F/He (1/9) as a reaction gas for the determination of As in biological fluids using a quadrupole ICP-MS instrument has been explored. A simple (dilute-and-shoot) interference-free method has been developed to quantify As concentrations at trace and ultra-trace levels in matrices with a high Cl content. As+ reacts with CH3F (through CH3F addition, followed by HF elimination) with high efficiency forming AsCH2 + as the primary reaction product, which can be monitored at a mass-to-charge ratio of 89, free from the Cl-based interferents (e.g., 40Ar35Cl+ and 40Ca35Cl+) that hamper the monitoring of 75As+. Matrix effects are overcome by the use of Te as an internal standard and the addition of 3% v/v ethanol to all samples and calibration standard solutions. The method presented was validated by analysing a set of reference materials (blood, serum and urine) and by assessing As recovery from a set of real blood samples. With this method, the limit of detection was calculated to be 0.8 ng L-1 As, favourably comparable to the vast majority of values reported in the literature, even with those obtained using more sophisticated sector-field instrumentation.

Details

Language :
English
Database :
OpenAIRE
Journal :
Zaguán. Repositorio Digital de la Universidad de Zaragoza, instname
Accession number :
edsair.dedup.wf.001..7afe6d7de6396ce92b7b095fcdb8641e