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Differential expression of heparan sulfate proteoglycans in cerebrovascular amyloid beta deposits in Alzheimer's disease and hereditary cerebral hemorrhage with amyloidosis (Dutch) brains
- Source :
- Acta Neuropathologica, 102, 6, pp. 604--14, Acta Neuropathologica, 102, 604--14
- Publication Year :
- 2001
-
Abstract
- Item does not contain fulltext Cerebrovascular deposition of amyloid beta protein (A beta) is a characteristic lesion of Alzheimer's disease (AD) and hereditary cerebral hemorrhage with amyloidosis of the Dutch type (HCHWA-D). Besides A beta, several other proteins and proteoglycans accumulate in cerebral amyloid angiopathy (CAA). We have now analyzed the expression of the heparan sulfate proteoglycan (HSPG) subtypes agrin, perlecan, glypican-1, syndecans 1-3 and HS glycosaminoglycan (GAG) side chains in CAA in brains of patients with AD and HCHWA-D. Hereto, specific well-characterized antibodies directed against the core protein of these HSPGs and against the GAG side chains were used for immunostaining. Glypican-1 was abundantly expressed in CAA both in AD and HCHWA-D brains, whereas perlecan and syndecans-1 and -3 were absent in both. Colocalization of agrin with vascular A beta was clearly observed in CAA in HCHWA-D brains, but only in a minority of the AD cases. Conversely, syndecan-2 was frequently associated with vascular A beta in AD, but did not colocalize with vascular A beta deposits in HCHWA-D. The three different syndecans, agrin, glypican-1 and HS GAG, but not perlecan, were associated with the majority of senile plaques (SPs) in all brains. Our results suggest a role for agrin in the formation of SPs and of CAA in HCHWA-D, but not in the pathogenesis of CAA in AD. Both syndecan-2 and glypican, but not perlecan, may be involved in the formation of CAA. We conclude that specific HSPG species may be involved in the pathogenesis of CAA in both AD and HCHWA-D, and that the pathogenesis of CAA and SPs may differ with regard to the involvement of HSPG species.
- Subjects :
- carbohydrates (lipids)
Disturbances in biochemical and functional development of the kidney during childhood
Immunologische ontstekingsprocessen in de nier
animal structures
Inflammatory reactions in the kidneys
mental disorders
nutritional and metabolic diseases
Pathofysiologie van Hersenen en Gedrag
cardiovascular diseases
Inborn errors of metabolism
Pathophysiology of Brain and Behaviour
Stoornissen in de biochemische en functionele ontwikkeling van de nier op kinderleeftijd
Erfelijke stofwisselingsziekten
Subjects
Details
- ISSN :
- 00016322
- Database :
- OpenAIRE
- Journal :
- Acta Neuropathologica, 102, 6, pp. 604--14, Acta Neuropathologica, 102, 604--14
- Accession number :
- edsair.dedup.wf.001..79b2060b4222e8443a5a8904a90ff2a4